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Step 1- Diagnosis Blood Test for Vitamin B12: This test determines the level of cobalamin (B12) in the blood

Following an overnight incubation at 4C in blocking buffer containing Gpa-GSS17 antibody at a dilution of 1 in 200, nematodes were washed, reblocked for 30 mins and incubated with FITC-conjugated goat anti-rabbit 2 o antibody (Sigma) at a dilution of 1 in 200 for 2h at room temperature

Immunopathology and C4d were reported in the lungs of six fatal cases in this age group, indicating that antibody-dependent complement activation through immune-complex formation may have been a contributing factor

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They work less effectively