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Following primary antibody incubation, sections were rinsed several times in 0.02M KPBS, incubated for 2 hr at room temperature in blocking buffer containing secondary antibodies against rabbit (raised in goat) conjugated with Alexa-Fluor fluorochromes (Life Technologies, Carlsbad, CA) and coverslipped using ProLong mounting medium (Life Technologies, Carlsbad, CA)