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Immunoreactivity assays for endogenous DSIP-like material in tissue and CSF have used antibodies whose specificity for the nonapeptide versus related peptides has not always been rigorously established (Kovalzon & Strekalova, 2006)

Human pancreatic stellate cell (hPSC) culture and treatments HPSCs (Fenghui Biotechnology Co., Ltd., Changsha, China) were cultured in RPMI 1640 medium (Gibco) with glucose (5.5 mmol/L glucose) supplemented with 10% FBS (Gibco) in an incubator with 5% CO2 at 37 C