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Unacylated ghrelin stimulates fatty acid oxidation to protect skeletal muscle against palmitate-induced impairment of insulin action in lean but not high-fat fed rats

The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO, 100 kDa) and subjected to size-exclusion chromatography (SEC) on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20mM HEPES pH 7.4, 100mM NaCl, 2mM MgCl 2 , 1M peptide, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS to separate complex from contaminants

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Am J Physiol Endocrinol Metab 2004

These findings underscore that not only rare pathogenic mutations, but also common variants exert modifying and additive effects on disease trajectories (Chung et al., 2023