Upon reaching 80% confluency, cells were washed with cold PBS and mechanically scraped into 1 mL of ice-cold Chelex-100-treated PBS containing 0.05% v/v benzonase (Sigma
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BPC 157 reduced levels of all necrosis markers, CK, CK-MB, LDH, and cTnT, and attenuated gross (no visible infarcted area) and histological damage, ECG (no ST-T ischemic changes), and echocardiography (preservation of systolic left ventricular function) damage induced by isoprenaline
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