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Briefly, total RNA was extracted using TRIzol reagent (Takara, Shiga, Japan)

4.3.2 Hypoxia Adipose tissue hypoxia is causally related to obesity-induced IR, especially in high-fat diet (HFD) fed and early obese patients, as adipocyte respiration becomes uncoupled, resulting in a state of increased oxygen consumption and relative adipocyte hypoxia (180)

Summary: Vitamin B12 pills do not cause weight loss in people with normal B12 levels, and no UK clinical guidance including NICE or NHS recommendations supports their use as a weight management intervention

Nature 510: 298302

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