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Lentivirus was generated by transfecting HEK293T cells plated to roughly 60% confluency in a six-well dish with 250 ng of pMD2.G, 750 ng of psPAX2 and 1,000 ng of lentiviral vector containing the gene of interest with 10 l of PEI in serum-free medium

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The LR3 IGF-I/IGF-1 protein, structurally and functionally akin to insulin, boasts significantly heightened growth-promoting activity compared to its counterpart

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