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I dont fancy that much of my time in a gym if Im honest with my last few weeks of uni coming up and basically wanna trim off the love handles so I dont look like a total sack of wet dogshit when we go on holiday in a few months

For immunostainings cells were blocked for 30 min in blocking buffer (10% GS, 0.3% Triton-X-100, 20 mM HEPES pH 7.4), then incubated with anti-p62 antibody (1:200, BD Bioscience, 610832), washed three times for 10 min in PBS, incubated with AF568-labeled goat-anti-mouse antibody, washed again three times in PBS for 10 min, and finally mounted using Shandon TM Immu-Mount TM containing Hoechst33342

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Leptin is another key hormonal metabolic regulator that is perturbed in obesity

Hazan S, Spradling-Reeves KD, Papoutsis A, Walker SJ