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Int J Biochem Cell Biol 41(8-9):16651677 Pires VC, Gollcke APB, Ribeiro DA, Lungato L, D'Almeida V, Aguiar O (2013) Grape juice concentrate protects reproductive parameters of male rats against cadmium-induced damage: a chronic assay

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Brains were decapitated, removed from the skull, cryoprotected in graded sucrose buffered solutions of 10%, 20%, and 30%, and flash-frozen at -65C using a cryostat