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Slides were deparaffinized and rehydrated on board prior to pre-treatments using Epitope Retrieval Solution 2 (Leica Biosystems) at 88C for 15 minutes, and ACD Enzyme from the Multiplex Reagent kit at 40C for 15 minutes

This pulsatile GH output then promotes hepatic and peripheral IGF1 production, supporting anabolic processes such as protein synthesis, lipolysis, and tissue repair

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The percentage change in body weight was similar across baseline BMI class subgroups and across mean medication doses