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10.1136/bmj-2023-075230 55 TurckD.TurckD.BohnT.CastenmillerJ.de HenauwS.Hirsch-ErnstK

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coli BL21(DE3) containing plasmid pANT43 was inoculated from an overnight preculture at a 1:100 ratio in four 2 l baffled Erlenmeyer flasks filled with 400 ml TB medium containing 50 g ml 1 kanamycin sulfate and incubated at 37 C, 140 rpm using a 5 cm shaking diameter for 2.75 h